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Associations between tissue fatty acid composition and physiological traits of performance and metabolism in the seabass (Dicentrarchus labrax) ArchiMer
Chatelier, Aurelien; Mckenzie, David; Prinet, Aurelie; Galois, Robert; Robin, Jean; Zambonino, Jose-luis; Claireaux, Guy.
Seabass were fed for 4 months with diets where the lipid was provided as either canola oil (CO), palm oil ( PO) or fish oil (FO), to generate diversity in their tissue fatty acid (FA) composition and investigate how this influenced major traits of exercise performance, cardiac performance and respiratory metabolism. In particular, based upon previous observations, we investigated the hypothesis that enriching the fish tissues with oleic and linoleic acids (OA, 18:1n-9 and LA, 18:2n-6, respectively) from the CO and PO diets would improve maximum exercise and cardiac performance, and increase aerobic metabolic scope. This proved to be the case; exercise respirometry on bass fitted with cardiac flow probes revealed that those fed CO and PO diets had a...
Tipo: Text Palavras-chave: Diet; Fatty acid; Cardiovascular performance; Metabolism; Swimming; Dicentrarchus labrax; Seabass.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1860.pdf
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Effects of oleic acid on the high threshold barium current in seabass Dicentrarchus labrax ventricular myocytes ArchiMer
Chatelier, Aurelien; Imbert, Nathalie; Zambonino, Jose-luis; Mckenzie, David; Bois, P.
The present study employed a patch clamp technique in isolated seabass ventricular myocytes to investigate the hypothesis that oleic acid (OA), a mono-unsaturated fatty acid, can exert direct effects upon whole-cell barium currents. Acute application of free OA caused a dose-dependent depression of the whole-cell barium current that was evoked by a voltage step to 0 mV from a holding potential of -80 mV. The derived 50% inhibitory concentration (IC50) was 12.49 +/- 0.27 mu mol l(-1). At a concentration of 30 mu mol l(-1), OA significantly reduced the current density to about 45% of control values, but did not modify either the shape of the current-density voltage relationship or the apparent reversal potential. In addition, OA did not modify the voltage...
Tipo: Text Palavras-chave: Fatty acid; Sea bass; Ventricular myocyte; L type calcium channel; Oleic acid.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-2120.pdf
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Linking swimming performance, cardiac pumping ability and cardiac anatomy in rainbow trout ArchiMer
Claireaux, Guy; Mckenzie, David; Genge, A; Chatelier, Aurelien; Aubin, Joel; Farrell, Anthony.
We exploited the inherent individual diversity in swimming performance of rainbow trout Oncorhynchus mykiss to investigate the hypothesis that maximum cardiac performance is linked to active metabolic rate (AMR) and critical swimming speed (U-crit). Six hundred juveniles (body mass similar to 150 g) were screened using a swimming challenge of 1.2 m s(-1) to identify 'poor swimmers' and 'good swimmers', i.e. the first and last 60 fish to fatigue, respectively. These 120 fish were individually tagged and then reared in common tanks for 9 months, where they grew at similar rates and achieved a similar body mass of approximately 1100 g. Critical swimming speed (U-crit) was then measured individually in tunnel respirometers, with simultaneous recordings of...
Tipo: Text Palavras-chave: Cardiac anatomy; Cardiac rythm; Swimming activity; Trout.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-420.pdf
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Modulation des performances cardio-respiratoires du bar europeen (Dicentrarchus labrax) par l'apport d'acides gras alimentaires: une etude integree des effets cardiaques in vivo au courant calcique de type L des myocytes ventriculaires ArchiMer
Chatelier, Aurelien.
Diet quality, and particularly fatty acids composition, is an environmental factor that is emerging as a significant source of physiological diversity. Indeed, numerous recent studies show that the fatty acid composition of the diet influences cardiac and metabolic performances of organisms. Because it is involved in the control and regulation of internal flow of matter and energy, the heart is believed to play a central role in environmental adaptation in fish. Within the double context of adaptation of individuals to their ecological niche and species evolution, understanding sources of interindividual variability and particularly fatty acid influences on the cardiorespiratory system is essential. The objective of the study was to investigate in the sea...
Tipo: Text Palavras-chave: Dicentrarchus labrax; Arythmie cardiaque; Hypoxie; Acide oléique; Canal calcique de type L; Calcium; Nage; Métabolisme; Coeur; Acides gras; Dicentrarchus labrax; Cardiac arrhythmias; Hypoxia; Oleic acid; L type calcium channel; Calcium; Metabolism; Heart; Fatty acid.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-2197.pdf
Registros recuperados: 4
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